In vitro Cytotoxic and Antimicrobial Activity of Essential Oil From Satureja Intermedia

BACKGROUND Many members of the genus Satureja have aromatic and medicinal characteristics. Objectives. OBJECTIVES The purpose of the present work was to determine cytotoxic activity of the essential oil of S. intermedia CA Mey (Lamiaceae) on two human cancerous cell lines and its in vitro inhibitory effects against 11 pathogenic bacteria and fungi as well. MATERIALS AND METHODS The essential oil was isolated by hydrodistillation and analyzed by combination of capillary GC-FID and GC-MS. The in vitro toxicological study was based on the MTT cytotoxicity assay and antimicrobial activity of the essential oil was studied according to the disc diffusion method and MIC value. RESULTS Thymol (34.5%), γ-terpinene (18.2%) and ρ-cymene (10.5%) were the main components of the essential oil. The toxicological study on 5637 and KYSE cell lines showed IC50 values of 156 μg/ml. The essential oil exhibited considerable antimicrobial activity on tested bacteria and fungi. CONCLUSIONS From the results of the present study, it may be concluded that the essential oil of S. intermedia and its major constitutes are interesting in antibacterial and anticancer applications.